Composite

Part:BBa_K2372009

Designed by: Marcel Chris Conrady   Group: iGEM17_Tuebingen   (2017-10-26)


pRha-RBS-BLS for controlled expression of beta-lactam synthetase

Our composite part was designed by combining pRha (Part: BBa_K914003), a Ribosome Binding Site (RBS) (Part: BBa_B0034) and BLS (Part: BBa_K2372002). The promoter pRha is used to control the expression of the target gene. The RBS is part of the mRNA and binds upstream at the 5’ End to the DNA. A RBSs task is to position the mRNAat the right place. The efficiency of the bonding determines how well the translation is accomplished. β-lactam Synthetase (BLS) catalyzes the formation of a β-lactam ring in clavulanic acid, which is a β-lactamase inhibitor. β-Lactamase is believed to be one of the main reasons of antibiotic resistance. Clavulanic acid has proven effective against a wide range of antibiotic resistant organisms.

References
https://www.nature.com/articles/nsb0801_684
http://www.pnas.org/content/95/16/9082.long
https://parts.igem.org/Part:BBa_K914003
https://parts.igem.org/Part:BBa_B0034


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 157
    Illegal AgeI site found at 347
    Illegal AgeI site found at 527
    Illegal AgeI site found at 692
    Illegal AgeI site found at 1184
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 779
    Illegal BsaI site found at 1372


[edit]
Categories
Parameters
None